anti mouse gitrl  (R&D Systems)

Journal: Immunology letters

Article Title: Increased expression of membrane-bound TGF-β1, GITR, and GITR ligand in patients with autoimmune thyroid disease.

doi: 10.1016/j.imlet.2025.107036

Figure Lengend Snippet: Fig. 4. Immunohistochemical staining of thyroid tissue from healthy subjects (A and D), AITD patient with GD (B and E), and HD (C and F) with anti-GITRL antibody (D, E, and F) and isotype controls (A, B, and C). GITRL is visualized using diaminobenzidine (brown) and counterstained with hematoxylin. GITRL expression is depicted on thyroid follicular cells. Arrows indicate GITRL expression on infiltrating cells. Magnification ×400.

Article Snippet: For the working dilution, the mouse anti-GITRL polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was diluted at 1:150.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Immunology letters

Article Title: Increased expression of membrane-bound TGF-β1, GITR, and GITR ligand in patients with autoimmune thyroid disease.

doi: 10.1016/j.imlet.2025.107036

Figure Lengend Snippet: Fig. 5. Histoscores of GITRL expression on thyroid follicular cells from patients with AITD (GD [black circle] and HD [white circle]) and healthy subjects. Wilcoxon test is used to compare differences in histoscores between AITD pa tients and healthy subjects.

Article Snippet: For the working dilution, the mouse anti-GITRL polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was diluted at 1:150.

Techniques: Expressing

Journal: Immunology letters

Article Title: Increased expression of membrane-bound TGF-β1, GITR, and GITR ligand in patients with autoimmune thyroid disease.

doi: 10.1016/j.imlet.2025.107036

Figure Lengend Snippet: Fig. 6. Serum titers of (A) TgAb or (B) McAb in AITD patients (GD [black circle] and HD [white circle]) with each histoscore of GITRL expression on thyroid follicular cells. Mann–Whitney U test is used to compare differences in TgAb or McAb between patients with AITD showing diverse histoscores.

Article Snippet: For the working dilution, the mouse anti-GITRL polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was diluted at 1:150.

Techniques: Expressing, MANN-WHITNEY

Journal: Neuro-oncology Advances

Article Title: GITRL-armed Delta-24-RGD oncolytic adenovirus prolongs survival and induces anti-glioma immune memory

doi: 10.1093/noajnl/vdz009

Figure Lengend Snippet: Antibodies and Their Conditions Used for Each Assay

Article Snippet: , Alexa Fluor® 647 rat anti-mouse GITRL , MIH44 , 1:100 , BD Biosciences , 563542.

Techniques: Plasmid Preparation, Western Blot, Flow Cytometry

Journal: The Journal of investigative dermatology

Article Title: Identification of Glucocorticoid-Induced TNF Receptor-Related Protein Ligand on Keratinocytes: Ligation by GITR Induces Keratinocyte Chemokine Production and Augments T-Cell Proliferation

doi: 10.1038/jid.2009.163

Figure Lengend Snippet: Skin punch biopsies obtained from (a) male Balb/C mice and (b) male C57Bl/6 mice were immunostained with anti-GITRL Ab (right panel, green) or with control IgG (left panel). The sections were counterstained with DAPI (blue). Representative data out of three independent experiments is shown (bar = 10 μm).

Article Snippet: Rabbit anti-mouse GITRL Ab, goat anti-mouse TARC Ab (clone N-20), and NF-κB p50 (clone D-17) Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Control

Journal: The Journal of investigative dermatology

Article Title: Identification of Glucocorticoid-Induced TNF Receptor-Related Protein Ligand on Keratinocytes: Ligation by GITR Induces Keratinocyte Chemokine Production and Augments T-Cell Proliferation

doi: 10.1038/jid.2009.163

Figure Lengend Snippet: Keratinocytes regulate the expression of TARC (a and b), CTACK (c and d), MCP-1 (e and f), and MBD3 (g and h) mRNA levels after reverse stimulation through GITRL. Skin biopsies from male Balb/C mice were incubated for 6 hours (a, c, e, and g) or 24 hours (b, d, f, and h) in the presence or absence of GITR: Fc FP (1 or 10 μg ml−1) or Control: Fc FP, then harvested and analyzed for mRNA expression by RT-PCR using primers specific for TARC, CTACK, MCP-1, MBD3, and 18S RNA. Values indicate the mean±SEM of six independent experiments.

Article Snippet: Rabbit anti-mouse GITRL Ab, goat anti-mouse TARC Ab (clone N-20), and NF-κB p50 (clone D-17) Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Expressing, Incubation, Control, Reverse Transcription Polymerase Chain Reaction

Journal: The Journal of investigative dermatology

Article Title: Identification of Glucocorticoid-Induced TNF Receptor-Related Protein Ligand on Keratinocytes: Ligation by GITR Induces Keratinocyte Chemokine Production and Augments T-Cell Proliferation

doi: 10.1038/jid.2009.163

Figure Lengend Snippet: (a) Cells were stained using PE-conjugated anti-GITRL Ab before and after fixing with 1% paraformaldehyde after overnight culture and analyzed by flow cytometry. The histogram represents one of five independent experiments. The black histogram denotes anti-GITRL Ab and the blue histogram denotes the isotype. (b) Cells were stained with PE-conjugated anti-GITRL Ab (red) and DAPI (blue) and analyzed by immunofluorescence (bar = 20 μm). (c) PAM 212 cells were incubated with GITR: Fc FP (1 or 10 μg ml−1) or with Control: Fc FP (10 μg ml−1) for 24 hours. Supernatants were harvested and TARC production was quantified by ELISA. Values indicate the mean±SEM of six independent experiments.

Article Snippet: Rabbit anti-mouse GITRL Ab, goat anti-mouse TARC Ab (clone N-20), and NF-κB p50 (clone D-17) Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Staining, Flow Cytometry, Immunofluorescence, Incubation, Control, Enzyme-linked Immunosorbent Assay

Journal: The Journal of investigative dermatology

Article Title: Identification of Glucocorticoid-Induced TNF Receptor-Related Protein Ligand on Keratinocytes: Ligation by GITR Induces Keratinocyte Chemokine Production and Augments T-Cell Proliferation

doi: 10.1038/jid.2009.163

Figure Lengend Snippet: (a) Cells were stained using both directly and indirectly FITC-conjugated anti-GITRL Ab and analyzed by flow cytometry. The histogram represents one of five independent experiments. The black histogram denotes anti-GITRL Ab and the blue histogram denotes the isotype. (b) Cells were stained with FITC-conjugated anti-GITRL Ab (green) and DAPI (blue) and analyzed by immunofluorescence (bar = 20 μm). HEKs regulate the expression of (c) CTACK (d) and IL-8 mRNA level after reverse stimulation through GITRL. Human keratinocytes were incubated for 6 hours in the presence or absence of GITR: Fc FP (1 or 10 μg ml−1) or Control: Fc FP, then harvested and analyzed for mRNA expression by RT-PCR using primers specific for CTACK, IL-8, and 18S RNA. Values indicate the mean±the SEM of three independent experiments.

Article Snippet: Rabbit anti-mouse GITRL Ab, goat anti-mouse TARC Ab (clone N-20), and NF-κB p50 (clone D-17) Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Staining, Flow Cytometry, Immunofluorescence, Expressing, Incubation, Control, Reverse Transcription Polymerase Chain Reaction

Journal: The Journal of investigative dermatology

Article Title: Identification of Glucocorticoid-Induced TNF Receptor-Related Protein Ligand on Keratinocytes: Ligation by GITR Induces Keratinocyte Chemokine Production and Augments T-Cell Proliferation

doi: 10.1038/jid.2009.163

Figure Lengend Snippet: Murine CD4+ T cells were isolated from mouse spleens and stimulated with anti-CD3 Ab in the presence or absence of keratinocytes. (a) Keratinocytes (1 × 104 well) enhance T-cell proliferation at increasing concentrations of anti-CD3 Ab (0.01–0.5 μg ml−1). (b) Keratinocytes were cultured with anti-GITRL blocking Ab (1 μg ml−1) or with mouse IgG1 isotype control for 1 hour before their use in proliferation assays.

Article Snippet: Rabbit anti-mouse GITRL Ab, goat anti-mouse TARC Ab (clone N-20), and NF-κB p50 (clone D-17) Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Isolation, Cell Culture, Blocking Assay, Control